In the field of biochemical research, buffering agents perform a very important function. The Tris buffers widely used today have a primary amino group and they are known to frequently cause inhibition problems in biological systems. Furthermore sufficient buffering power cannot be obtained under pH 7.5.
Good and co-workers have developed buffers to overcome the above-noted defects and their superiority has been indicated by the Hill reactions. These buffers are referred to as Good's Buffers being named after the inventor.

[Characteristics]
1) Acid dissociation constant pKa is between 6 ~ 8.
2) Solubility in water is high.
3) Will not penetrate through biomembranes and cell membranes.
4) Low base effect toward biological systems.
5) The acid dissociation constant is minimally affected by concentration, temperature and ion composition.
6) Low in ability to form complexes with metal ions.
7) Chemically stable.
8) Low in absorption of visible and ultra-violet rays.


Composition and pH range