Buffers

Good's Buffers

Instant Buffers

Buffers for Sequenation

Others

Related Reagent for Buffer

Good's Buffers

In the field of biochemical research, buffering agents perform a very important function. The Tris buffers widely used today have a primary amino group and they are known to frequently cause inhibition problems in biological systems. Furthermore sufficient buffering power cannot be obtained under pH7.5.
Good and co-workers have developed buffers to overcome the above-noted defects and their superiority has been indicated by the Hill reactions. These buffers are referred to as Good’s Buffers being named after the inventor.

[Characteristics]
1) Acid dissociation constant pKa is between 6~8.
2) High water solubility.
3) Low penetration through biomembranes.
4) Low base effect toward biological systems.
5) pKa is less affected by concentration, temperature and ion composition.
6) Low complexation ability with metal ions.
7) Chemically stable.
8) Low in absorption of visible and ultra-violet rays.

Composition and pH range

A0700 A0700 A0699 A0699 B0909 B0909
B1494 B1494 B1493 B1493 C0920 C0920
C0921 C0921 B0484 B0484 H0772 H0772
H0576 H0576 H0396 H0396 H0671 H0671
M0606 M0606 M0707 M0707 P1233 P1233
P1131 P1131 T0682 T0682 T0683 T0683
T1364 T1364 T0974 T0974
A0700 N-(2-Acetamido)-2-aminoethanesulfonic Acid [Good's buffer component for biological research]
A0699 N-(2-Acetamido)iminodiacetic Acid
B0909 N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic Acid [Good's buffer component for biological research]
B1494 3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic Acid
B1493 Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane [Good's buffer component for biological research]
C0920 2-Cyclohexylaminoethanesulfonic Acid [Good's buffer component for biological research]
C0921 3-Cyclohexylaminopropanesulfonic Acid [Good's buffer component for biological research]
B0484 N,N-Di(2-hydroxyethyl)glycine [Good's buffer component for biological research]
H0772 4-(2-Hydroxyethyl)piperazine-1-(2-hydroxypropane-3-sulfonic Acid) Hydrate [Good's buffer component for biological research]
H0576 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic Acid [Good's buffer component for biological research]
H0396 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid [Good's buffer component for biological research]
H0671 2-Hydroxy-3-morpholinopropanesulfonic Acid
M0606 2-Morpholinoethanesulfonic Acid
M0707 3-Morpholinopropanesulfonic Acid [Good's buffer component for biological research]
P1233 Piperazine-1,4-bis(2-ethanesulfonic Acid)
P1131 Piperazine-1,4-bis(2-hydroxypropanesulfonic Acid) Dihydrate [Good's buffer component for biological research]
T0682 Tricine [Good's buffer component for biological research]
T0683 N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid [Good's buffer component for biological research]
T1364 3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic Acid [Good's buffer component for biological research]
T0974 N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic Acid [Good's buffer component for biological research]
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Instant Buffers

Instant buffers are in granulated form. Dissolve immediately in purified water to give the desired buffer solution.

I0234 Instant Buffer K40 pH4.0 (acc. to Kolthoff) (50packs)
I0241 Instant Buffer S64 pH6.4 (acc. to Soerensen) (50packs)
I0243 Instant Buffer S70 pH7.0 (acc. to Soerensen) (50packs)
I0245 Instant Buffer S74 pH7.4 (acc. to Soerensen) (50packs)
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■Preparation

Buffers for Sequenation

Q0046 Q0046 Q0051 Q0051
Q0046 Quadrol Buffer [1M Quadrol-TFA Buffer in Propanol-Water (3/4 v/v, pH9.0)] [Sequenation Reagent]
Q0051 Quadrol Buffer [0.25M Quadrol-TFA Buffer in Propanol-Water (3/4 v/v, pH9.5)] [Sequenation Reagent]
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Others

B2904 Buffer Solution pH8.7 (6mol/L Guanidine Hydrochloride)
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Related Reagent for Buffer

B1057 B1057 G0124 G0124 I0288 I0288
L0165 L0165 M0713 M0713 P0874 P0874
P0875 P0875 P0309 P0309 T0025 T0025
T2516 T2516 T0740 T0740 T0962 T0962
B1057 1,3-Bis[tris(hydroxymethyl)methylamino]propane [for Buffer Material]
G0124 Glycylglycine
I0288 Imidazole [for Buffer]
L0165 L-Lactic Acid
M0713 N-Methyl-D-glucamine Hydrochloride [for Buffer]
P0874 Piperazine-1,4-bis(2-ethanesulfonic Acid) Disodium Salt [Good's buffer component for biological research]
P0875 Piperazine-1,4-bis(2-ethanesulfonic Acid) Monosodium Salt [Good's buffer component for biological research]
P0309 Potassium Hydrogen Phthalate
T0025 L-(+)-Tartaric Acid
T2516 Tris(hydroxymethyl)aminomethane [for Electrophoresis]
T0740 Tris(hydroxymethyl)aminomethane Hydrochloride
T0962 N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid Sodium Salt Hydrate [Good's buffer component for biological research]
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Literature

N. E. Good, G. D. Winget, W. Winter, T. N. Connolly, S. Izawa, R. M. M. Singh, Biochemistry 1966, 5, 467 [DOI]; W. J. Ferguson, K. I. Braunschweiger, W. R. Braunschweiger, J. R. Smith, J. J. McCormick, C. C. Wasmann, N. P. Jarvis, D. H. Bell, N. E. Good, Anal. Biochem. 1980, 104, 300 [DOI].

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