Labeling Reagents for HPLC

HPLC is utilized extensively as a means of detecting and determining trace components. Labeling objective substances for analysis with labeling reagents appropriate for detection methods, has been performed in order to obtain higher sensibility and selectivity. A great number of labeling reagents have been reported for this purpose. NBD- and DBD-derivatives are excellent fluorescent labeling reagents with strong long wave-length fluorescence resulting from their benzoxadiazole structure.
For electrochemical detectors ferrocene derivatives have been reported. Ferrocenes have been utilized as the most suitable labeling reagents for electrochemcial detectors because they respond to the application of relatively low voltages, such as +0.5 V, and they are excellent redox system of oxidation and reduction. N-(4-Aminobutyl)-N-ethylisoluminol has been reported to be usable as a chemiluminescence labeling reagent.

UV Detection

Fluorescence Detection

Chemiluminescence Detection

Electrochemical Detection

UV Detection

for Carboxyl Groups

B0949 B0949 A5501 A5501 B0633 B0633
A5502 A5502 A5503 A5503 A5504 A5504
A5506 A5506 A5505 A5505 M0815 M0815
A5507 A5507 A5508 A5508
B0949 1-Benzyl-3-p-tolyltriazene [for Esterification]
A5501 4-Bromophenacyl Bromide [for HPLC Labeling]
B0633 2-Bromo-4'-phenylacetophenone
A5502 9-Chloromethylanthracene [for HPLC Labeling]
A5503 N-Chloromethyl-4-nitrophthalimide [for HPLC Labeling]
A5504 N-Chloromethylphthalimide [for HPLC Labeling]
A5506 N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea [for HPLC Labeling]
A5505 3'-Methoxyphenacyl Bromide [for HPLC Labeling]
M0815 3'-Methoxyphenacyl Bromide
A5507 1-(4-Nitrobenzyl)-3-p-tolyltriazene [for HPLC Labeling]
A5508 Phenacyl Bromide [for HPLC Labeling]
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for Amino Groups

B0105 B0105 D1382 D1382 D1653 D1653
A5511 A5511 F0197 F0197 D2259 D2259
A5523 A5523 A5524 A5524 M0721 M0721
I0189 I0189 N0713 N0713 N0714 N0714
I0125 I0125 I0190 I0190 I0015 I0015
N0176 N0176 A5513 A5513 S0004 S0004
A5522 A5522 A5514 A5514 A5515 A5515
B0105 Benzoyl Chloride
D1382 Dabsyl Chloride [N-Protecting Agent for Peptides Research]
D1653 4-(Dimethylamino)azobenzene 4'-Isothiocyanate
A5511 3,5-Dinitrobenzoyl Chloride [for HPLC Labeling]
F0197 9-Fluorenylmethyl Chloroformate [N-Protecting Agent for Peptides Research]
D2259 Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-alaninamide [HPLC Labeling Reagent for e.e. Determination]
A5523 Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide [HPLC Labeling Reagent for e.e. Determination]
A5524 Nalpha-(5-Fluoro-2,4-dinitrophenyl)-D-leucinamide [HPLC Labeling Reagent for e.e. Determination]
M0721 4-Methoxybenzoyl Chloride
I0189 Methyl Isothiocyanate
N0713 (1R,2R)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0714 (1S,2S)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
I0125 1-Naphthyl Isocyanate
I0190 1-Naphthyl Isothiocyanate
I0015 Ninhydrin
N0176 4-Nitrobenzoyl Chloride
A5513 Phenyl Isothiocyanate [for HPLC Labeling]
S0004 Salicylaldehyde
A5522 N-Succinimidyl 4-Nitrophenylacetate [for HPLC Labeling]
A5514 2,3,4,6-Tetra-O-acetyl-beta-D-glucopyranosyl Isothiocyanate [for HPLC Labeling]
A5515 2,3,4,6-Tetra-O-benzoyl-beta-D-glucopyranosyl Isothiocyanate [for HPLC Labeling]
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for Hydroxyl Groups

B0105 B0105 A5511 A5511 M0721 M0721
N0713 N0713 N0714 N0714 N0176 N0176
B0105 Benzoyl Chloride
A5511 3,5-Dinitrobenzoyl Chloride [for HPLC Labeling]
M0721 4-Methoxybenzoyl Chloride
N0713 (1R,2R)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0714 (1S,2S)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0176 4-Nitrobenzoyl Chloride
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for Carbonyl Groups

A5533 A5533 D0845 D0845 A5531 A5531
A5532 A5532
A5533 Dabsyl Hydrazine [for HPLC Labeling]
D0845 2,4-Dinitrophenylhydrazine (wetted with ca. 50% Water, containing 25g and 500g on a dry weight basis respectively)
A5531 2,4-Dinitrophenylhydrazine Hydrochloride [for HPLC Labeling]
A5532 O-4-Nitrobenzylhydroxylamine Hydrochloride [for HPLC Labeling]
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Fluorescence Detection

for Carboxyl Groups

A5576 A5576 A0979 A0979 A5551 A5551
B1926 B1926 A5570 A5570 A5553 A5553
A5502 A5502 A5560 A5560 A5561 A5561
A5574 A5574 A5571 A5571 A5555 A5555
A5562 A5562 A5563 A5563 A5573 A5573
A5554 A5554
A5576 AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]
A0979 7-Acetoxy-4-bromomethylcoumarin [for HPLC Labeling]
A5551 Br-Mmc (=4-Bromomethyl-7-methoxycoumarin) [for HPLC Labeling]
B1926 9-(Bromomethyl)acridine [for HPLC Labeling]
A5570 4-Bromomethyl-6,7-dimethoxycoumarin [for HPLC Labeling]
A5553 3-Bromomethyl-7-methoxy-1,4-benzoxazin-2-one [for HPLC Labeling]
A5502 9-Chloromethylanthracene [for HPLC Labeling]
A5560 (S)-(+)-DBD-APy [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5561 (R)-(-)-DBD-APy [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5574 DBD-ED [=4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5571 DBD-CO-Hz [=4-(N,N-Dimethylaminosulfonyl)-7-(N-hydrazinocarbonylmethyl-N-methyl)amino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5555 DBD-PZ [=4-(N,N-Dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5562 (S)-(+)-NBD-APy [=(S)-(+)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5563 (R)-(-)-NBD-APy [=(R)-(-)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5573 NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5554 NBD-PZ (=4-Nitro-7-piperazino-2,1,3-benzoxadiazole) [for HPLC Labeling]
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for Amino Groups

A1657 A1657 A1658 A1658 D0005 D0005
D0656 D0656 A5558 A5558 A5595 A5595
A5575 A5575 A5564 A5564 A5565 A5565
A5568 A5568 A5569 A5569 D3178 D3178
A5579 A5579 F0197 F0197 F0192 F0192
F0026 F0026 M0722 M0722 A5594 A5594
N0713 N0713 N0714 N0714 A5592 A5592
A5572 A5572 A5593 A5593 A5566 A5566
A5567 A5567 A5577 A5577 A5578 A5578
P0280 P0280 S0503 S0503
A1657 (1R,2R)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A1658 (1S,2S)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
D0005 Dansyl Chloride (10% in Acetone)
D0656 Dansyl Chloride
A5558 DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5595 DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5575 DBD-NCS [=4-(N,N-Dimethylaminosulfonyl)-7-isothiocyanato-2,1,3-benzoxadiazole] [for HPLC Labeling and Edman Degradation]
A5564 (S)-(-)-DBD-Pro-COCl [=(S)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5565 (R)-(+)-DBD-Pro-COCl [=(R)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5568 (R)-(-)-DBD-Py-NCS [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5569 (S)-(+)-DBD-Py-NCS [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
D3178 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoic Acid
A5579 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl Chloride Hydrochloride [for HPLC Labeling]
F0197 9-Fluorenylmethyl Chloroformate [N-Protecting Agent for Peptides Research]
F0192 Fluorescamine [for HPLC Labeling]
F0026 Fluorescein Isothiocyanate (isomer I)
M0722 2-Methoxy-2,4-diphenyl-3(2H)-furanone
A5594 2,3-Naphthalenedialdehyde [Fluorimetric reagent for primary Amines]
N0713 (1R,2R)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0714 (1S,2S)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A5592 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
A5572 NBD-COCl [=4-(N-Chloroformylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5593 NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
A5566 (R)-(+)-NBD-Pro-COCl [=(R)-(+)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5567 (S)-(-)-NBD-Pro-COCl [=(S)-(-)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5577 (R)-(-)-NBD-Py-NCS [=(R)-(-)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5578 (S)-(+)-NBD-Py-NCS [=(S)-(+)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
P0280 o-Phthalaldehyde [for HPLC Labeling]
S0503 Succinimidyl 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoate
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for Hydroxyl Groups

A0411 A0411 A1657 A1657 A1658 A1658
D0005 D0005 D0656 D0656 A5558 A5558
A5564 A5564 A5565 A5565 D3178 D3178
A5579 A5579 N0713 N0713 N0714 N0714
A5572 A5572 A5566 A5566 A5567 A5567
A0411 2-Aminopyridine
A1657 (1R,2R)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A1658 (1S,2S)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
D0005 Dansyl Chloride (10% in Acetone)
D0656 Dansyl Chloride
A5558 DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5564 (S)-(-)-DBD-Pro-COCl [=(S)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5565 (R)-(+)-DBD-Pro-COCl [=(R)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
D3178 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoic Acid
A5579 4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl Chloride Hydrochloride [for HPLC Labeling]
N0713 (1R,2R)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0714 (1S,2S)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A5572 NBD-COCl [=4-(N-Chloroformylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5566 (R)-(+)-NBD-Pro-COCl [=(R)-(+)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5567 (S)-(-)-NBD-Pro-COCl [=(S)-(-)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
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for Carbonyl Groups

A0267 A0267 A5581 A5581 A5552 A5552
A5556 A5556 A5571 A5571 A5557 A5557
A5573 A5573
A0267 2-Aminobenzenethiol
A5581 1,3-Cyclohexanedione [for HPLC Labeling]
A5552 Dansyl Hydrazine [for HPLC Labeling]
A5556 DBD-H [=4-(N,N-Dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5571 DBD-CO-Hz [=4-(N,N-Dimethylaminosulfonyl)-7-(N-hydrazinocarbonylmethyl-N-methyl)amino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5557 NBD-H (=4-Hydrazino-7-nitro-2,1,3-benzoxadiazole Hydrazine) [for HPLC Labeling]
A5573 NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
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for Mercapto Groups

A5597 A5597 A5596 A5596 A5595 A5595
A5591 A5591 A5592 A5592 A5593 A5593
P1214 P1214
A5597 ABD-F [=4-(Aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for Determination of Thiols]
A5596 DAABD-Cl [=4-[2-(Dimethylamino)ethylaminosulfonyl]-7-chloro-2,1,3-benzoxadiazole] [for Proteome Analysis]
A5595 DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5591 NAM [=N-(9-Acridinyl)maleimide] [for HPLC Labeling]
A5592 NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
A5593 NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
P1214 N-(1-Pyrenyl)maleimide
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Chemiluminescence Detection

A5304 A5304 D2339 D2339
A5304 N-(4-Aminobutyl)-N-ethylisoluminol [Chemiluminescence Reagent]
D2339 2,3-Dihydro-6-isothiocyanato-1,4-phthalazinedione [HPLC Labeling Reagent for Chemiluminescent]
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Electrochemical Detection

F0406 F0406 F0280 F0280 H0941 H0941
S0820 S0820 S0599 S0599
F0406 Ferroceneacetic Acid
F0280 Ferroceneboronic Acid (contains varying amounts of Anhydride) [Cyclic boronating reagent for GC/MS]
H0941 (Hydrazinocarbonyl)ferrocene [for HPLC Labeling]
S0820 N-Succinimidyl Ferrocenecarboxylate
S0599 Succinimidyl (2R)-6-(Tetrahydro-2H-pyran-2-yloxy)-2,5,7,8-tetramethylchroman-2-carboxylate
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